GrippeFranceV1, Main, Exploration, bibRecord, 000107

Usefulness of multiplex PCR methods and respiratory viruses' distribution in children below 15 years old according to age, seasons and clinical units in France: A 3 years retrospective study.

Identifieur interne : 000107 ( Main/Exploration ); précédent : 000106; suivant : 000108

Usefulness of multiplex PCR methods and respiratory viruses' distribution in children below 15 years old according to age, seasons and clinical units in France: A 3 years retrospective study.

Auteurs : Benoit Visseaux [France] ; Gilles Collin [France] ; Houria Ichou [France] ; Charlotte Charpentier [France] ; Samia Bendhafer [France] ; Madalina Dumitrescu [France] ; Lahcene Allal [France] ; Bogdan Cojocaru [France] ; Luc Desfrère [France] ; Diane Descamps [France] ; Laurent Mandelbrot [France] ; Nadhira Houhou-Fidouh [France]

Source :

PloS one [ 1932-6203 ] ; 2017.

RBID : pubmed:28235002

Descripteurs français

KwdFr :
- Adolescent (MeSH), Enfant (MeSH), Enfant d'âge préscolaire (MeSH), Femelle (MeSH), France (MeSH), Humains (MeSH), Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Infections à virus respiratoire syncytial (diagnostic), Maladies virales (diagnostic), Mâle (MeSH), Nourrisson (MeSH), Partie nasale du pharynx (virologie), Réaction de polymérisation en chaine multiplex (méthodes), Saisons (MeSH), Virus respiratoires syncytiaux (isolement et purification), Études rétrospectives (MeSH).
MESH :
- diagnostic : Infections de l'appareil respiratoire, Infections à virus respiratoire syncytial, Maladies virales.
- isolement et purification : Virus respiratoires syncytiaux.
- méthodes : Réaction de polymérisation en chaine multiplex.
- virologie : Infections de l'appareil respiratoire, Partie nasale du pharynx.
- Adolescent, Enfant, Enfant d'âge préscolaire, Femelle, France, Humains, Mâle, Nourrisson, Saisons, Études rétrospectives.

English descriptors

KwdEn :
- Adolescent (MeSH), Child (MeSH), Child, Preschool (MeSH), Female (MeSH), France (MeSH), Humans (MeSH), Infant (MeSH), Male (MeSH), Multiplex Polymerase Chain Reaction (methods), Nasopharynx (virology), Respiratory Syncytial Virus Infections (diagnosis), Respiratory Syncytial Viruses (isolation & purification), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (virology), Retrospective Studies (MeSH), Seasons (MeSH), Virus Diseases (diagnosis).
MESH :
- diagnosis : Respiratory Syncytial Virus Infections, Respiratory Tract Infections, Virus Diseases.
- isolation & purification : Respiratory Syncytial Viruses.
- methods : Multiplex Polymerase Chain Reaction.
- virology : Nasopharynx, Respiratory Tract Infections.
- Adolescent, Child, Child, Preschool, Female, France, Humans, Infant, Male, Retrospective Studies, Seasons.

Abstract

BACKGROUND

To date, only influenza and RSV testing are recommended for respiratory viruses' detection in paediatric units. In this study, we described, according to seasons, ages and clinical units, the results obtained in children (<15 years old) by multiplex-PCR (mPCR) tests allowing a quick and wide range detection of all respiratory viruses. These results were also compared with RSV specific detection.

METHODS

All nasopharyngeal mPCR and RSV tests requested by clinicians in our French teaching hospitals group between 2011 and 2014 were retrospectively included. All repeated samples for the same children in the same month were discarded.

RESULTS

Of the 381 mPCR tests (344 children) performed, 51.4% were positive. Positivity and viral co-infection rates were higher in the 6-36 months old strata (81% and 25%, p<0.0001 and p = 0.04, respectively). Viral distribution showed strong variations across ages. During specific influenza epidemic periods, only 1/39 (2.5%) mPCR tests were positive for influenza and 19/39 (48.7%) for other viruses. During specific RSV epidemic periods, only 8/46 (17.4%) mPCR tests were positive for RSV and 14/46 (30.4%) for other viruses. 477/1529 (31.2%) of RSV immunochromatography-tests were positive. Among the negatives immunochromatography-test also explored by mPCR, 28/62 (31%) were positive for other respiratory viruses.

CONCLUSION

This study provides a wide description of respiratory viruses' distribution among children in hospital settings using mPCR over 3 years. It emphasizes the number of undiagnosed respiratory viruses according to the current diagnosis practice in France and gives a better picture of respiratory viruses identified in hospital settings by mPCR all over the year in France.

DOI: 10.1371/journal.pone.0172809
PubMed: 28235002
PubMed Central: PMC5325537

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000125
to stream Main, to step Curation: 000125

Le document en format XML

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<author><name sortKey="Bendhafer, Samia" sort="Bendhafer, Samia" uniqKey="Bendhafer S" first="Samia" last="Bendhafer">Samia Bendhafer</name>
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<author><name sortKey="Dumitrescu, Madalina" sort="Dumitrescu, Madalina" uniqKey="Dumitrescu M" first="Madalina" last="Dumitrescu">Madalina Dumitrescu</name>
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<author><name sortKey="Allal, Lahcene" sort="Allal, Lahcene" uniqKey="Allal L" first="Lahcene" last="Allal">Lahcene Allal</name>
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<author><name sortKey="Cojocaru, Bogdan" sort="Cojocaru, Bogdan" uniqKey="Cojocaru B" first="Bogdan" last="Cojocaru">Bogdan Cojocaru</name>
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<author><name sortKey="Desfrere, Luc" sort="Desfrere, Luc" uniqKey="Desfrere L" first="Luc" last="Desfrère">Luc Desfrère</name>
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<author><name sortKey="Descamps, Diane" sort="Descamps, Diane" uniqKey="Descamps D" first="Diane" last="Descamps">Diane Descamps</name>
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<country xml:lang="fr">France</country>
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<author><name sortKey="Mandelbrot, Laurent" sort="Mandelbrot, Laurent" uniqKey="Mandelbrot L" first="Laurent" last="Mandelbrot">Laurent Mandelbrot</name>
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<author><name sortKey="Houhou Fidouh, Nadhira" sort="Houhou Fidouh, Nadhira" uniqKey="Houhou Fidouh N" first="Nadhira" last="Houhou-Fidouh">Nadhira Houhou-Fidouh</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent (MeSH)</term>
<term>Child (MeSH)</term>
<term>Child, Preschool (MeSH)</term>
<term>Female (MeSH)</term>
<term>France (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Infant (MeSH)</term>
<term>Male (MeSH)</term>
<term>Multiplex Polymerase Chain Reaction (methods)</term>
<term>Nasopharynx (virology)</term>
<term>Respiratory Syncytial Virus Infections (diagnosis)</term>
<term>Respiratory Syncytial Viruses (isolation & purification)</term>
<term>Respiratory Tract Infections (diagnosis)</term>
<term>Respiratory Tract Infections (virology)</term>
<term>Retrospective Studies (MeSH)</term>
<term>Seasons (MeSH)</term>
<term>Virus Diseases (diagnosis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent (MeSH)</term>
<term>Enfant (MeSH)</term>
<term>Enfant d'âge préscolaire (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>France (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Infections de l'appareil respiratoire (diagnostic)</term>
<term>Infections de l'appareil respiratoire (virologie)</term>
<term>Infections à virus respiratoire syncytial (diagnostic)</term>
<term>Maladies virales (diagnostic)</term>
<term>Mâle (MeSH)</term>
<term>Nourrisson (MeSH)</term>
<term>Partie nasale du pharynx (virologie)</term>
<term>Réaction de polymérisation en chaine multiplex (méthodes)</term>
<term>Saisons (MeSH)</term>
<term>Virus respiratoires syncytiaux (isolement et purification)</term>
<term>Études rétrospectives (MeSH)</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Respiratory Syncytial Virus Infections</term>
<term>Respiratory Tract Infections</term>
<term>Virus Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections de l'appareil respiratoire</term>
<term>Infections à virus respiratoire syncytial</term>
<term>Maladies virales</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Respiratory Syncytial Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus respiratoires syncytiaux</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Multiplex Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaine multiplex</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term>
<term>Partie nasale du pharynx</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nasopharynx</term>
<term>Respiratory Tract Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Child</term>
<term>Child, Preschool</term>
<term>Female</term>
<term>France</term>
<term>Humans</term>
<term>Infant</term>
<term>Male</term>
<term>Retrospective Studies</term>
<term>Seasons</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term>
<term>Enfant</term>
<term>Enfant d'âge préscolaire</term>
<term>Femelle</term>
<term>France</term>
<term>Humains</term>
<term>Mâle</term>
<term>Nourrisson</term>
<term>Saisons</term>
<term>Études rétrospectives</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>To date, only influenza and RSV testing are recommended for respiratory viruses' detection in paediatric units. In this study, we described, according to seasons, ages and clinical units, the results obtained in children (<15 years old) by multiplex-PCR (mPCR) tests allowing a quick and wide range detection of all respiratory viruses. These results were also compared with RSV specific detection.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>All nasopharyngeal mPCR and RSV tests requested by clinicians in our French teaching hospitals group between 2011 and 2014 were retrospectively included. All repeated samples for the same children in the same month were discarded.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Of the 381 mPCR tests (344 children) performed, 51.4% were positive. Positivity and viral co-infection rates were higher in the 6-36 months old strata (81% and 25%, p<0.0001 and p = 0.04, respectively). Viral distribution showed strong variations across ages. During specific influenza epidemic periods, only 1/39 (2.5%) mPCR tests were positive for influenza and 19/39 (48.7%) for other viruses. During specific RSV epidemic periods, only 8/46 (17.4%) mPCR tests were positive for RSV and 14/46 (30.4%) for other viruses. 477/1529 (31.2%) of RSV immunochromatography-tests were positive. Among the negatives immunochromatography-test also explored by mPCR, 28/62 (31%) were positive for other respiratory viruses.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>This study provides a wide description of respiratory viruses' distribution among children in hospital settings using mPCR over 3 years. It emphasizes the number of undiagnosed respiratory viruses according to the current diagnosis practice in France and gives a better picture of respiratory viruses identified in hospital settings by mPCR all over the year in France.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28235002</PMID>
<DateCompleted><Year>2017</Year>
<Month>09</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>02</Month>
<Day>08</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>12</Volume>
<Issue>2</Issue>
<PubDate><Year>2017</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS ONE</ISOAbbreviation>
</Journal>
<ArticleTitle>Usefulness of multiplex PCR methods and respiratory viruses' distribution in children below 15 years old according to age, seasons and clinical units in France: A 3 years retrospective study.</ArticleTitle>
<Pagination><MedlinePgn>e0172809</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0172809</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">To date, only influenza and RSV testing are recommended for respiratory viruses' detection in paediatric units. In this study, we described, according to seasons, ages and clinical units, the results obtained in children (<15 years old) by multiplex-PCR (mPCR) tests allowing a quick and wide range detection of all respiratory viruses. These results were also compared with RSV specific detection.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">All nasopharyngeal mPCR and RSV tests requested by clinicians in our French teaching hospitals group between 2011 and 2014 were retrospectively included. All repeated samples for the same children in the same month were discarded.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Of the 381 mPCR tests (344 children) performed, 51.4% were positive. Positivity and viral co-infection rates were higher in the 6-36 months old strata (81% and 25%, p<0.0001 and p = 0.04, respectively). Viral distribution showed strong variations across ages. During specific influenza epidemic periods, only 1/39 (2.5%) mPCR tests were positive for influenza and 19/39 (48.7%) for other viruses. During specific RSV epidemic periods, only 8/46 (17.4%) mPCR tests were positive for RSV and 14/46 (30.4%) for other viruses. 477/1529 (31.2%) of RSV immunochromatography-tests were positive. Among the negatives immunochromatography-test also explored by mPCR, 28/62 (31%) were positive for other respiratory viruses.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This study provides a wide description of respiratory viruses' distribution among children in hospital settings using mPCR over 3 years. It emphasizes the number of undiagnosed respiratory viruses according to the current diagnosis practice in France and gives a better picture of respiratory viruses identified in hospital settings by mPCR all over the year in France.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Visseaux</LastName>
<ForeName>Benoit</ForeName>
<Initials>B</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-9279-5538</Identifier>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Collin</LastName>
<ForeName>Gilles</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ichou</LastName>
<ForeName>Houria</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Charpentier</LastName>
<ForeName>Charlotte</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bendhafer</LastName>
<ForeName>Samia</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Service de Néonatologie, Hôpital Louis Mourier, AP-HP, Colombes, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dumitrescu</LastName>
<ForeName>Madalina</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Service des Urgences pédiatrique, Hôpital Louis Mourier, AP-HP, Colombes, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Allal</LastName>
<ForeName>Lahcene</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Service de Maternité, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cojocaru</LastName>
<ForeName>Bogdan</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Service des Urgences pédiatrique, Hôpital Louis Mourier, AP-HP, Colombes, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Desfrère</LastName>
<ForeName>Luc</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Service de Néonatologie, Hôpital Louis Mourier, AP-HP, Colombes, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Descamps</LastName>
<ForeName>Diane</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mandelbrot</LastName>
<ForeName>Laurent</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Service de Maternité, Hôpital Louis Mourier, AP-HP, Colombes, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Houhou-Fidouh</LastName>
<ForeName>Nadhira</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>02</Month>
<Day>24</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
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<CitationSubset>IM</CitationSubset>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName>
</MeshHeading>
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<MeshHeading><DescriptorName UI="D005602" MajorTopicYN="N">France</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060885" MajorTopicYN="N">Multiplex Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018357" MajorTopicYN="N">Respiratory Syncytial Virus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012136" MajorTopicYN="N">Respiratory Syncytial Viruses</DescriptorName>
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<Month>11</Month>
<Day>15</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
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<Day>09</Day>
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<affiliations><list><country><li>France</li>
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<tree><country name="France"><region name="Île-de-France"><name sortKey="Visseaux, Benoit" sort="Visseaux, Benoit" uniqKey="Visseaux B" first="Benoit" last="Visseaux">Benoit Visseaux</name>
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<name sortKey="Charpentier, Charlotte" sort="Charpentier, Charlotte" uniqKey="Charpentier C" first="Charlotte" last="Charpentier">Charlotte Charpentier</name>
<name sortKey="Cojocaru, Bogdan" sort="Cojocaru, Bogdan" uniqKey="Cojocaru B" first="Bogdan" last="Cojocaru">Bogdan Cojocaru</name>
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<name sortKey="Descamps, Diane" sort="Descamps, Diane" uniqKey="Descamps D" first="Diane" last="Descamps">Diane Descamps</name>
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Usefulness of multiplex PCR methods and respiratory viruses' distribution in children below 15 years old according to age, seasons and clinical units in France: A 3 years retrospective study.

Usefulness of multiplex PCR methods and respiratory viruses' distribution in children below 15 years old according to age, seasons and clinical units in France: A 3 years retrospective study.

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